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目的：了解缺氧复氧对培养新生大鼠心肌细胞超微结构的影响及其黄芪皂甙的保护作用。方法：实验细胞分为三组：对照组（以Ｈａｎｋ液代替培养液培养）、损伤组（以氮饱和无氧无糖Ｈａｎｋ液密闭培养３ｈ后复氧）及保护组（Ｈａｎｋ液中加入２５ｍｇ·Ｌ－１黄芪皂甙，余同损伤组）用电镜观察各组超微结构。结果：电镜下，损伤组心肌细胞的细胞形态、细胞膜、细胞核、线粒体等结构均可发生明显的改变，而黄芪皂甙保护组心肌细胞超微结构的改变则明显较损伤组轻。结论：黄芪皂甙具有减轻实验性缺氧复氧对培养的心肌细胞损伤的作用。
Objective: To investigate the effect of hypoxia-reoxygenation on the ultrastructure of cultured neonatal rat cardiomyocytes and the protective effect of astragaloside. Methods: The experimental cells were divided into three groups: the control group (Hank liquid instead of culture medium), the injury group (reoxygenated after nitrogen saturation and oxygen free sugar-free Hank liquid confinement culture for 3h) and the protective group (Hank liquid added 25mg · L-1 astragaloside, with the same injury group) electron microscopy ultrastructure. Results: Under electron microscope, the cell morphology, cell membrane, nucleus, mitochondria and other structures of myocardial cells in injured group could be changed obviously. The ultrastructure changes of myocardial cells in astragaloside protective group were obviously lighter than that in injured group. Conclusion: Astragaloside can alleviate the injury of cultured cardiomyocytes induced by hypoxia and reoxygenation.