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为了探讨女用口服避孕药对细胞膜阳离子转移酶的影响,作者研究了服药妇女红细胞膜的Na~+-K~+ATP酶及Mg~(++)-Ca~(++)ATP酶活性。37名18～35岁的研究对象中对照9名,孕妇18名(孕期在30～36周)。另有10名给服d-18甲基炔诺酮150微克和炔雌醇30微克或50微克,连续13～15个周期。比较3组妇女红细胞内及血浆中Na~+、K~+含量及红细胞膜的Na~+-K~+ATP酶和Mg~(++)-Ca~(++)ATP酶活性。后一测定为取空腹静脉血按Nakao等氏法制备红细胞膜,并于24小时内进行膜的酶活性测定,酶的活性单位以每小时释放pi微克分子数确定,此放射活性以每毫克膜蛋白所含酶的单位数表示。同时用火焰光度计测定红细胞内和血浆中的Na~+、K~+含量。结果表明,女用口服避孕药对红细
In order to investigate the effect of oral contraceptives on the activity of cell membrane cationic transferase, the authors studied Na ~ + -K ~ + ATPase and Mg ~ (++) - Ca ~ (++) ATPase activities of erythrocyte membrane in women taking the drug. Among 37 subjects aged 18-35 years, 9 were controls and 18 were pregnant women (30-36 weeks of pregnancy). Another 10 were given d-18 norgestrel 150 micrograms and ethinyl estradiol 30 micrograms or 50 micrograms for 13 to 15 cycles. The contents of Na ~ + and K ~ + in erythrocytes and plasma and the activities of Na ~ + -K ~ + ATPase and Mg ~ (++) - Ca ~ (++) ATPase in erythrocyte membrane were compared between the three groups. The latter was determined to take fasting venous blood by Nakao et al. Preparation of erythrocyte membrane, and within 24 hours for the determination of enzyme activity, the enzyme activity unit to determine the release of pi micrograms per hour, the radioactivity per milligram of membrane The number of units of protein contained in the protein. At the same time, the content of Na ~ + and K ~ + in erythrocytes and plasma was determined by flame photometer. The results show that female oral contraceptives red fine