论文部分内容阅读
目的研究淫羊藿低糖苷组分(LGFEH)对UMR-106细胞和波尼松龙诱导的骨质疏松斑马鱼的作用。方法将不同质量浓度的LGFEH与UMR-106细胞共同培养后,MTT法测定LGFEH对UMR-106细胞增殖的影响,通过测定细胞内外碱性磷酸酶(ALP)活性评价LGFEH对细胞分化的影响。将受精后3 d的斑马鱼幼鱼分为空白培养基组、0.5%DMSO溶媒对照组、泼尼松龙组、依替膦酸二钠组、LGFEH(1、2、4、8、16μg/m L)组。每天换液至受精后9 d,处死,采用茜素红对各组斑马鱼幼鱼骨骼染色,并以显微检测、数码成像方法定量分析骨骼染色区域。结果 LGFEH不仅可以促进成骨样细胞UMR-106细胞的增殖,促进其合成和分泌ALP,而且能够显著增加斑马鱼头部骨骼染色面积(矿化面积)和染色累积吸光度值(骨密度)。结论 LGFEH体内及体外具有较好的抗骨质疏松作用。
Objective To investigate the effect of Epimedium glycoside fraction (LGFEH) on osteoporosis zebrafish induced by UMR-106 cells and prednisolone. Methods Different concentrations of LGFEH and UMR-106 cells were co-cultured. The effect of LGFEH on the proliferation of UMR-106 cells was determined by MTT assay. The effect of LGFEH on the differentiation of UMR-106 cells was evaluated by measuring the activity of ALP. The zebrafish juveniles 3 days after fertilization were divided into blank medium group, 0.5% DMSO vehicle control group, prednisolone group, etidronate disodium group, LGFEH (1,2,4,8,16μg / m L) group. Every day the liquid was changed to 9 days after fertilization, and sacrificed. Alizarin red was used to stain the bones of zebrafish juveniles in each group, and the bone staining area was quantitatively analyzed by microscopic examination and digital imaging. Results LGFEH not only promoted the proliferation of osteoblast-like cells UMR-106 cells, promoted its synthesis and secretion of ALP, but also significantly increased the skeletal stained area (mineralized area) and the cumulative absorbance value (BMD) of zebrafish head. Conclusion LGFEH has good anti-osteoporosis effect both in vitro and in vivo.