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目的:筛选一种适合分离纯化枇杷花总黄酮的大孔吸附树脂并确定其最佳工艺参数。方法:以总黄酮的吸附率与解析率为指标,从D101、AB-8、DM301、S-8四种大孔吸附树脂中筛选出最佳树脂,并通过单因素考察该树脂分离纯化总黄酮的最佳工艺条件。结果:DM301树脂对枇杷花总黄酮具有良好的吸附分离性能,最佳工艺参数为:上样浓度12.5 mg/mL,上样流速2 mL/min,上样pH 5.0,树脂最大上样量60 mg/g;最佳洗脱剂50%乙醇,洗脱速率3 mL/min,洗脱剂用量10 BV。结论:在所确定的工艺条件下,DM301树脂分离纯化枇杷花总黄酮效果良好,以Al(NO3)3-NaNO2-NaOH比色法计,产物经干燥后总黄酮质量分数最高可以达到80%以上。
OBJECTIVE: To screen a macroporous resin suitable for isolation and purification of total flavonoids from Eriobotrya japonica and to determine its optimum technological parameters. Methods: The optimum resin was screened from four kinds of macroporous adsorption resins, D101, AB-8, DM301 and S-8 with the adsorption rate and resolution ratio of total flavonoids. The single flavonoids The best process conditions. Results: DM301 resin had good adsorption and separation properties for the total flavonoids of Eriobotrya japonica. The optimal parameters were as follows: sample concentration 12.5 mg / mL, sample flow rate 2 mL / min, sample loading 5.0, resin maximum loading 60 mg / g; the best eluent 50% ethanol, the elution rate of 3 mL / min, the amount of eluent 10 BV. Conclusion: Under the determined conditions, DM301 resin has good effect on the isolation and purification of total flavonoids from Eriobotrya japonica. The content of total flavonoids after drying can reach more than 80% by Al (NO3) 3-NaNO2-NaOH colorimetry .