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背景:苯是重要的工业溶剂,长期接触可导致苯可导致DNA损伤,染色体畸变,DNA加合物的形成,基因突变等。目的:了解苯对体内DNA的损伤作用、机制以及抗氧化酶体系的变化情况。设计:随机对照的实验研究。单位:哈尔滨医科大学附属第一医院检验科,哈尔滨医科大学公共卫生学院。材料:实验在哈尔滨医科大学公共卫生学院动物室完成。选用健康雄性昆明种小鼠24只,体质量18-22g,由哈尔滨医科大学附属第二医院实验动物中心提供。干预:分为正常对照组、低剂量苯损伤组和高剂量苯损伤组。除对照组外,其余各组小鼠进行静式吸入苯蒸气染毒,4h/d,2个月后处死,分离骨髓细胞及外周血淋巴细胞,取肝、脾、脑组织并制备匀浆。主要观察指标:用单细胞凝胶电泳技术对骨髓细胞及外周血淋巴细胞DNA损伤进行检测;同时检测肝、脾、脑组织中超氧化物歧化酶(superoxidedismu-lase,SOD)、谷胱肝肽过氧化物酶(glutathioneperoxidase,GSH-Px)的活性及丙二醛(malondialdehyde,MDA)含量。结果:染毒组小鼠骨髓细胞及外周血淋巴细胞彗星百分率分别为骨髓细胞(83.56±10.28)%,(92.54±15.93)%,外周血淋巴细胞(41.27±6.03)%,(65.79±11.62)%,显著高于对照组(4.13±0.52)%,(2.21±0.31)%(P<0.01),并呈剂量-反应关系。高、低浓度组小鼠肝匀浆SOD活?
Background: Benzene is an important industrial solvent. Long-term exposure to benzene can lead to DNA damage, chromosomal aberration, DNA adduct formation and gene mutation. Objective: To understand the effect of benzene on DNA damage in vivo mechanism and antioxidant enzyme system changes. Design: Randomized controlled experimental study. Unit: First Affiliated Hospital of Harbin Medical University Laboratory, Harbin Medical University School of Public Health. Materials: The experiment was completed in the animal room of Harbin Medical University School of Public Health. 24 healthy male Kunming mice were selected and their body weight was 18-22g, which was provided by Experimental Animal Center of the Second Affiliated Hospital of Harbin Medical University. Intervention: divided into normal control group, low-dose benzene injury group and high-dose benzene injury group. Except for the control group, the other mice in each group were exposed to benzene vapor inhalation. After 4h / d and 2 months, the mice were sacrificed and the bone marrow cells and peripheral blood lymphocytes were separated. The liver, spleen and brain tissues were harvested and homogenized. MAIN OUTCOME MEASURES: DNA damage of bone marrow cells and peripheral blood lymphocytes was detected by single cell gel electrophoresis. At the same time, superoxide dismutase (SOD), glutathione peptide Glutathioneperoxidase (GSH-Px) activity and malondialdehyde (MDA) content were measured. RESULTS: The percentages of comet cells in bone marrow cells and peripheral blood lymphocytes of mice in the treated group were (83.56 ± 10.28)%, (92.54 ± 15.93)%, (41.27 ± 6.03)%, (65.79 ± 11.62)%, %, Which was significantly higher than that in the control group (4.13 ± 0.52)% and (2.21 ± 0.31)%, respectively (P <0.01). High and low concentrations of liver homogenate SOD activity?