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目的:探讨尿激酶型纤溶酶原激活物(uPA)、纤溶酶原激活物抑制剂-1(PAI-1)在食管癌发生、发展中的作用及意义。方法:采用免疫组织化学SP法检测正常食管黏膜上皮组织(18例)、食管黏膜上皮不典型增生组织(23例)及食管癌组织(68例)中uPA、PAI-1蛋白的表达,并分析其和食管癌临床病理特征的关系。结果:uPA蛋白在正常食管黏膜上皮组织、食管黏膜上皮不典型增生组织及食管癌组织中的阳性率分别为27.8%、39.1%、70.6%,三者间有显著性差异(P<0.05);PAI-1蛋白在正常食管黏膜上皮组织、食管黏膜上皮不典型增生组织及食管癌组织中的阳性率分别为22.2%、34.8%、64.7%,三者间有显著性差异(P<0.05)。uPA、PAI-1蛋白表达与年龄、性别、病理类型无关(P>0.05),在临床病理Ⅰ-Ⅱ期和Ⅲ-Ⅳ期间有升高的趋势,但差异无统计学意义(P>0.05),与分化程度和淋巴结有无转移有关(P<0.05)。结论:uPA、PAI-1蛋白在食管癌发生、发展不同阶段的表达呈进行性上升的趋势,可能与食管癌的发生、发展相关,过度表达提示预后不良。
Objective: To investigate the role and significance of urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) in the pathogenesis of esophageal cancer. Methods: The expression of uPA and PAI-1 in normal esophageal epithelium (18 cases), esophageal epithelial dysplasia (23 cases) and esophageal cancer tissues (68 cases) were detected by immunohistochemical SP method Its relationship with clinicopathological features of esophageal cancer. Results: The positive rates of uPA protein in normal esophageal mucosa epithelial tissue, esophageal mucosa epithelial dysplasia tissue and esophageal cancer tissue were 27.8%, 39.1% and 70.6% respectively (P <0.05). The positive rates of PAI-1 protein in normal esophageal mucosa epithelial tissue, esophageal mucosa epithelial dysplasia tissue and esophageal cancer tissue were 22.2%, 34.8% and 64.7%, respectively (P <0.05). The expression of uPA and PAI-1 protein had no correlation with age, sex and pathological type (P> 0.05), but there was an increasing trend in clinical stageⅠ-Ⅱ and Ⅲ-Ⅳ, but the difference was not statistically significant (P0.05) , With the degree of differentiation and lymph node metastasis (P <0.05). CONCLUSION: The expressions of uPA and PAI-1 protein in esophageal carcinoma at different stages of development are progressively increasing, which may be related to the occurrence and development of esophageal carcinoma. Overexpression of uPA and PAI-1 may lead to poor prognosis.