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目的 研制载脂蛋白A1和B检测试剂。方法 采用硫酸葡聚糖沉淀、超速离心、层析等方法。结果 分离的载脂蛋白A1(ApoA1)和B (ApoB) ,经检定证实其为单一成分 ,用它们为抗原 ,免疫绵羊 ,获得ApoA1和ApoB抗血清 ,检定结果与德国Bochinger公司产品一致。用这两种抗血清配制的浊度检测试剂与德国Diasys公司的同类试剂盒进行对照 ,相关良好。结论 所研制的载脂蛋白A1和B检测试剂达到了国外同类产品的质量水平。
Objective To develop apolipoprotein A1 and B detection reagents. Methods Dextran sulfate precipitation, ultracentrifugation, chromatography and other methods. Results The isolated apolipoproteins A1 (ApoA1) and B (ApoB) were confirmed to be single components by the assay. Sheep were immunized with them to obtain ApoA1 and ApoB antisera. The results were in accordance with those of the German company Bochinger. The turbidimetric reagent formulated with both antisera was well-matched to similar Diasys kit from Germany. Conclusion The developed apolipoprotein A1 and B detection reagents have reached the quality level of similar foreign products.