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目的:研究不同剂量银杏叶(ginkgo biloba extract,EGb761)对Aβ诱导神经元损伤的影响。方法:取高、中、低剂量的EGb761质量浓度分别为100,10,1 mg·L-1预处理Aβ1-42干预的神经元,检测神经元活力、凋亡率及NeuN染色的变化。结果:与Aβ组相比,高和低剂量EGb761组的神经元活力和NeuN+细胞数存在显著差异(P<0.05),而中剂量EGb761组神经元活力和NeuN+细胞数均存在极显著差异(P<0.01);中剂量EGb761组的对神经元凋亡的保护作用优于高、低剂量EGb761组。结论:高、中、低剂量组银杏叶制剂均可提高损伤海马神经元活力、提高NeuN+细胞数,降低神经元凋亡率;尤以中剂量组显著。提示采用预处理的给药方式可保护Aβ诱导的神经元损伤。
Objective: To study the effects of ginkgo biloba extract (EGb761) on Aβ-induced neuronal injury. Methods: Neurons were treated with 100, 10, 1 mg · L-1 of high, medium and low doses of EGb761 pretreated with Aβ1-42 respectively. The viability, apoptosis rate and NeuN staining were measured. RESULTS: Compared with Aβ group, the neuronal activity and the number of NeuN + cells in high and low doses of EGb761 group were significantly different (P <0.05), while there were significant differences in the activity of neurons and NeuN + cells in EGb761 group <0.01). The protective effect of EGb761 at medium dose on neuronal apoptosis was better than that of high and low dose EGb761 group. CONCLUSION: Ginkgo biloba extract can increase the activity of neurons in hippocampus, increase the number of NeuN + cells and decrease the apoptosis rate of neurons in high, medium and low dose groups, especially in middle dose group. It is suggested that pretreatment administration can protect Aβ-induced neuronal injury.