论文部分内容阅读
目的观察壳聚糖-纳米羟基磷灰石(CS/nHA)复合制剂对粪肠球菌生物膜的体外抗菌作用,为研究该药物对根管内粪肠球菌生物膜抗菌的作用打下基础,探索激光共聚焦显微镜(CLSM)技术在研究生物膜领域中的优势。方法采用液体二倍稀释法,测定壳聚糖/纳米羟基磷灰石复合制剂对游离状态下粪肠球菌的最小抑菌浓度和最小杀菌浓度;将粪肠球菌在体外培养成生物膜后,利用CLSM观察研究该生物膜中细菌活性。结果 CS/nHA制剂在0.467 mg/mL时对游离状态下的粪肠球菌具有抑菌和杀菌作用,与对照组相比差异有统计学意义。使用CS/nHA制剂前后粪肠球菌生物膜活菌百分比显著下降,与对照组相比差异有统计学意义(P<0.05)。结论 CS/nHA制剂对粪肠球菌有较强的抗菌作用,将其作为根管封闭剂中的抗菌成分具有一定的应用前景。
Objective To observe the in vitro antibacterial activity of chitosan-nano-hydroxyapatite (CS / nHA) composite on Enterococcus faecalis biofilm and lay a foundation for studying the antibacterial effect of this drug on E. faecalis biofilm in root canal, The advantages of confocal microscopy (CLSM) technology in the field of biomembrane research. Methods The liquid two-fold dilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MIC) of chitosan / nano-hydroxyapatite in free state. After Enterococcus faecalis culture in vitro, CLSM observations of bacterial activity in this biofilm. Results CS / nHA preparation had bacteriostatic and bacteriostatic effects on Enterococcus faecalis in free state at 0.467 mg / mL, which was significantly different from the control group. The percentage of viable Enterococcus faecalis biofilm before and after CS / nHA preparation was significantly decreased compared with the control group (P <0.05). Conclusion The CS / nHA preparation has a strong antibacterial activity against Enterococcus faecalis, which has certain application prospects as an antibacterial component in root canal sealant.