论文部分内容阅读
目的了解某部驻区蜱传斑点热的自然疫源地情况。方法进行蜱类与啮齿动物调查,利用立氏立克次体190kD外膜蛋白A(R.rOmpA)基因序列设计引物,对683只蜱类标本和146个鼠类脏器标本进行PCR检测,随机抽取一株森林革蜱阳性扩增产物进行序列测定。结果调查地区存在3种蜱、5种啮齿动物;从森林革蜱、嗜群血蜱和啮齿动物脏器标本中检测斑点热立克次体DNA片段,阳性率分别为53.81%、7.41%和6.16%;所测序列与前苏联的DnS14株的同源性为100%,与我国检测的BJ90、HLJ054的同源性分别为88%和86%。结论调查地区存在与DnS14株型别一致的斑点热立克次体株。
Objective To understand the status of natural foci of tick-borne fever in a certain area. Methods The ticks and rodents were investigated. The primers of 190 rd RrmpA gene were used to detect 683 ticks and 146 murine organ samples. A positive forest goose tick amplification product was sequenced. Results There were 3 species of ticks and 5 species of rodents in the surveyed area. The positive rate of the detected DNA fragment of spotted fever rickettsia was 53.81%, 7.41% and 6.16 %. The homology between the tested sequence and DnS14 strain in the former Soviet Union was 100%. The homologies with the BJ90 and HLJ054 strains detected in our country were 88% and 86% respectively. Conclusions There is a strain of Dictyostelophus virgatus coexisting with DnS14 in the surveyed area.