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目的探讨线粒体呼吸链在高压氧(hyperbaric oxygen,HBO)诱导细胞活性氧产生中的作用。方法采用特异性荧光探针DHE和Mito-SOX分别标记全细胞和线粒体内超氧阴离子(O_2·~-),以荧光显微技术检测HBO暴露后人脐静脉内皮细胞(human umbilical vein endothelium cells,HUVEC)内O_2·~-的生成。结果 HBO暴露后全细胞O_2·~-升高(31±8)%(P<0.01),线粒体内O_2·~-升高(137±19)%(P<0.01);给予呼吸链复合体Ⅱ抑制剂TTFA后,两者O_2·~-的增长分别被抑制(55±11)%(P<0.05)和(61±8)%(P<0.01)。结论 HBO主要通过线粒体呼吸链增加HUVEC活性氧生成。
Objective To investigate the role of mitochondrial respiratory chain in the production of reactive oxygen species (ROS) induced by hyperbaric oxygen (HBO). Methods The whole cells and mitochondrial superoxide anion (O 2 ·· -) were labeled by specific fluorescent probes DHE and Mito-SOX, respectively. The expression of human umbilical vein endothelium cells (HUVECs) HUVEC) O_2 · ~ - generation. RESULTS: After exposure to HBO, the content of O 2 ·· - was increased (31 ± 8)% (P <0.01) and the content of O 2 · - in mitochondria was increased by 137 ± 19% (P <0.01) After the TTFA inhibitor, the increase of O 2 ·· - was inhibited by (55 ± 11)% (P <0.05) and (61 ± 8)% (P <0.01), respectively. Conclusion HBO increases HUVEC reactive oxygen species mainly through mitochondrial respiratory chain.