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从贻贝斧足肌中提取了多酚氧化酶和芳基硫酸酯酶,前者能作用于邻苯二酚和邻苯三酚,但不作用于对苯二酚;后者能作用于底物硝基邻苯二酚硫酸酯(P-NitrocatecholSulphate)。应用硫脲和NaHSO_3及KH_2PO_4和Na_2SO_3,均能抑制幼贝和成贝分泌足丝进行附着,抑制浓度分别是10—15mmol硫脲,2—4‰NaHSO_3;1—5mmol KH_2PO_4,1—3mmolNa_2SO_3,这种抑制作用是可逆的。本实验结果进一步证明贻贝足丝的形成过程是足丝酚蛋白的单宁化过程,同多酚氧化酶和芳基硫酸酯酶的作用有关。
Polyphenol oxidase and aryl sulfatase were extracted from Muscle Axillary Muscle, the former acting on catechol and pyrogallol, but not on hydroquinone; the latter acting on the substrate P-Nitrocatechol Sulphate. The application of thiourea and NaHSO_3 and KH_2PO_4 and Na_2SO_3 can inhibit the secretion of foot and silkworm shellfish adhesion, inhibitory concentrations were 10-15mmol thiourea, 2-4 ‰ NaHSO_3; 1-5mmol KH_2PO_4,1-3mmolNa_2SO_3, which The inhibition is reversible. The results of this experiment further prove that the formation of mussel foot silk is the process of tannin hydrolysis of foot-silk protein, with the role of polyphenol oxidase and aryl sulfatase.