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目的:分析血清miR-21在肝癌发生过程中的表达水平并将其与传统肝癌血清标志物甲胎蛋白(AFP)比较,探索其成为肝癌早期诊断血清标志物的可能性。方法:二乙基亚硝胺(DENA)腹腔注射诱导建立大鼠肝癌模型,建模过程中收集造癌各个阶段的血清。Realtime-PCR检测血清miR-21的表达情况,ELISA法检测血清AFP水平。结果:与正常组及纤维化期大鼠相比,miR-21在肝硬化期、肝癌早期、肝癌晚期的大鼠血清均有不同程度上调(P<0.05),AFP在肝癌早、晚期的大鼠血清明显上调(P<0.05);与肝硬化期大鼠比较,肝癌早、晚期大鼠体内的miR-21表达显著上调(P<0.05),AFP在肝癌早期、肝癌晚期均显著上调(P<0.05)。结论:血清miR-21参与了肝癌发生的过程,对于肝癌发生的各个阶段均有很大的指示作用,可能作为肝癌预防和早期诊断的一个潜在标志物。
OBJECTIVE: To analyze the expression level of miR-21 in hepatocellular carcinoma (HCC) and to compare it with AFP, a serum marker of traditional hepatocellular carcinoma (HCC), and to explore its potential as an early diagnostic marker of HCC. Methods: Rat model of hepatocellular carcinoma was established by intraperitoneal injection of diethylnitrosamine (DENA). The serum of all stages of carcinogenesis was collected during the modeling. Realtime-PCR was used to detect the expression of miR-21 in serum. Serum AFP level was detected by ELISA. Results: Compared with the normal group and the fibrosis stage, miR-21 was up-regulated in cirrhosis, early stage of liver cancer and late stage of liver cancer (P <0.05) (P <0.05). Compared with cirrhotic rats, the expression of miR-21 in early and late stage of hepatocellular carcinoma was significantly increased (P <0.05), and the level of AFP in early stage of liver cancer and late stage of liver cancer were significantly increased (P <0.05). Conclusion: Serum miR-21 is involved in the process of hepatocarcinogenesis and plays a significant role in all stages of hepatocellular carcinoma. It may serve as a potential biomarker for the prevention and early diagnosis of hepatocellular carcinoma.