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The existence of multiple adenylate cy- clase encoding genes implies the importance of cAMP in Sinorhizobium meliloti 1021. In this study, as a pioneer step of understanding cAMP roles, mi- croarray analysis on S. meliloti was carried out for the function of exogenous cAMP. To our surprise, the result showed that the transcriptions of glnII and glnK genes were significantly upshifted in the presence of exogenous cAMP in S. meliloti. This phenomenon is further confirmed in S. meliloti that the expression of either glnII or glnK promoter-lacZ translational fusion is higher in the presence of exogenous cAMP. Therefore, for the first time, we have identified genes from S. meliloti whose expression is activated by cAMP. The potential physiological role of upregula- tion of glnII and glnK by cAMP is discussed.
The existence of multiple adenylate cy-clase encoding genes implies the importance of cAMP in Sinorhizobium meliloti 1021. In this study, as a pioneer step of understanding cAMP roles, mi- croarray analysis on S. meliloti was carried out for the function of exogenous cAMP To our surprise, the result showed that the transcriptions of glnII and glnK genes were significantly upshifted in the presence of exogenous cAMP in S. meliloti. This phenomenon is further confirmed by S. meliloti that the expression of either glnII or glnK promoter-lacZ translational fusion is higher in the presence of exogenous cAMP. we have identified genes from S. meliloti whose expression is activated by cAMP. The potential physiological role of upregula tion of glnII and glnK by cAMP is discussed.