利用透射电镜观察了放线菌酮体内诱导大鼠脾细胞凋亡的形态学变化,结果显示,腹腔注射放线菌酮4小时后,大鼠脾细胞发生凋亡,凋亡脾细胞核和胞质发生一系列形态学变化.主要表现为胞核染色质浓缩、重排、呈不同形态;多数凋亡脾细胞的粗面内质网增殖,凋亡小体形成,其线粒体也大量增加,或单个分布或团聚,肿胀、空泡化.结果提示,凋亡脾细胞的主要细胞器主动参与凋亡过程. Transmission electron microscopy was used to observe the morphological changes of splenocytes induced by actinomycin in vivo. The results showed that after 4 hours of intraperitoneal injection of cycloheximide, apoptosis of splenocytes and apoptosis of splenocytes were observed A series of morphological changes occurred, mainly manifested as chromatin condensation, rearrangement, showing different morphology; most of the apoptotic spleen cells proliferation of rough endoplasmic reticulum, the formation of apoptotic bodies, the mitochondria also increased significantly, or a single Distribution or reunion, swelling, vacuolization.The results suggest that the major organelles of apoptotic splenocytes are actively involved in the apoptotic process.