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目的探讨桂皮提取物桂皮酸对胰腺癌细胞系BxPC-3细胞增殖和凋亡的作用,并探讨其可能的作用机制。方法以胰腺癌BxPC-3细胞为研究对象,用不同浓度(0~8mmol·L~(-1))桂皮酸分别处理24、48、72h后,CCK-8比色法检测细胞活力;桂皮酸(0、1、2、4mmol·L~(-1))处理BxPC-3细胞48h后,PI/AnnexinⅤ检测细胞凋亡率,Western blot法检测药物对细胞Bax、Bcl-2、PTEN、Akt、p-Akt蛋白表达的影响。结果桂皮酸能有效抑制BxPC-39细胞增殖,且呈浓度、时间依赖性;桂皮酸处理BxPC-3细胞48h后,细胞凋亡率增加;促凋亡蛋白Bax表达量上调,而抗凋亡蛋白Bcl-2表达量下调;此外,桂皮酸可上调PTEN蛋白表达,并下调Akt蛋白磷酸化。结论桂皮酸可抑制BxPC-3细胞增殖并诱导凋亡,其作用机制可能与上调PTEN蛋白表达并抑制Akt蛋白磷酸化,继而上调Bax蛋白,下调Bcl-2蛋白相关。
Objective To investigate the effect of cinnamic acid, cinnamon extract, on proliferation and apoptosis of pancreatic cancer cell line BxPC-3 and its possible mechanism. Methods Pancreatic cancer BxPC-3 cells were treated with different concentrations of cinnamic acid (0 ~ 8 mmol·L -1) for 24, 48 and 72 h, respectively. Cell viability was measured by CCK-8 colorimetric assay. Cinnamic acid The apoptosis rate of BxPC-3 cells was detected by PI / AnnexinⅤ assay after treated with 0, 1, 2, 4mmol·L -1 for 48 hours. The expressions of Bax, Bcl-2, PTEN, Akt, Effect of p-Akt protein expression. Results Cinnamic acid could effectively inhibit the proliferation of BxPC-39 cells in a concentration-and time-dependent manner. After treated with cinnamic acid for 48 hours, the apoptosis rate of BxPC-3 cells increased, while that of pro-apoptotic protein Bax was up-regulated. Bcl-2 expression was down-regulated. In addition, cinnamic acid upregulated PTEN protein expression and down-regulated Akt protein phosphorylation. Conclusion Cinnamic acid can inhibit the proliferation and induce the apoptosis of BxPC-3 cells. The mechanism may be related to up-regulating the expression of PTEN protein and inhibiting the phosphorylation of Akt protein, then up-regulating Bax protein and down-regulating Bcl-2 protein.