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生长素在调控植物根系发育上起着重要的作用。本研究利用根系特异表达的磷转运蛋白AtPht1;1的启动子驱动生长素合成基因OsYUCCA1,构建表达载体,通过农杆菌介导法转入水稻品种中花11,获得转基因阳性植株;根据Os YUCCA1基因在水稻根中的表达量高低将转基因植株分为强表达株系和弱表达株系。转基因植株与野生型对照相比,根系构型变化明显,不定根、侧根和根毛数目均增加,主根长度变短。强表达转基因株系的地上部分的生长发育也受到影响。我们的研究结果表明弱表达株系根系构型变化适中且地上部分生长不受影响,具有实际应用的潜力。
Auxin plays an important role in the regulation of plant root development. In this study, OsYUCCA1, an auxin biosynthesis gene, was driven by a root-specific transporter of AtPht1; a promoter was constructed and inserted into Zhonghua 11 by Agrobacterium tumefaciens-mediated transfection to obtain transgenic plants. According to the Os YUCCA1 gene Transgenic plants were divided into strong and weakly expressed lines in rice roots. Compared with the wild-type control, the transgenic plants showed obvious changes in root configuration. The number of adventitious roots, lateral roots and root hairs increased, and the length of main roots became shorter. The growth and development of the aerial parts of strongly expressed transgenic lines are also affected. Our results show that the root architecture of the weakly-expressing lines is moderately changed and the growth of aboveground parts is unaffected, which has the potential of practical application.