单克隆抗体(MCAs)被认为是生物技术化时代的新兴明星。自1975年发现单克隆抗体以来,它们已成为生物技术化时代的关键性研究工具,开发着许多新的生物学和医学研究领域。经免疫动物产生的多克隆抗血清,多年来用于识别、纯化及测定各种分子(酶、药物及激素)以及作为几种不同类型细胞的标记物。然而,在不同时间,由不同动物所得的多克隆抗血清存在着批间误差。与之相比,由产生抗体的细胞单克隆所产生出的MCAs则能达到最佳纯度及无限量产生。如果需要,使杂交瘤细胞在无血清的环境中生 Monoclonal antibodies (MCAs) are considered emerging stars in the biotech era. Since the discovery of monoclonal antibodies in 1975, they have become a key research tool in the age of biotechnology and have developed many new fields of biology and medical research. The polyclonal antisera produced by immunized animals have been used for many years to identify, purify and assay various molecules (enzymes, drugs and hormones) and as markers for several different types of cells. However, at different times, there are inter-assay errors in polyclonal antisera obtained from different animals. In contrast, MCAs produced by monoclonal antibody-producing cells produce optimal purity and yield in an unlimited amount. Hybridoma cells are allowed to germinate in a serum-free environment if required