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对日本鳗鲡(Anguillajaponica)卵巢发育的时间与催产率的关系进行研究,对卵母细胞在体内胚泡破裂(GVBD)的发生过程、以及用17α-OHP和17α,20β-DHP诱导日本鳗鲡排卵的方式、时机和催产效果进行观察,结果表明,卵巢发育时间越长,催产率越低。造成催产率低的原因主要是由于日本鳗鲡在卵母细胞成熟后期,雌鳗个体对催产药物敏感性差异较大,催产时机难以掌握。通过采用适合日本鳗鲡卵母细胞的透明液观察GVBD的发生过程,并根据每个个体GVBD的发生速度,制订适宜的催产方案基本解决了上述难题。本实验采用CP、HCG、17α-OHP或17α,20β-DHP混合催产,使其平均催产率由35 4%分别提高到91 9%和93 0%。17α-OHP和17α,20β-DHP的催产剂量为5mg/(500g)和10mg/(500g)。在(21±0 5)℃水温条件下,催产效应时间分别为15～18h和13～16h。
The relationship between the development of ovary and the rate of oxytocin in Japanese eel (Anguilla japonica) was studied. The development of oocysts in vivo in blastocyst rupture (GVBD) and the induction of ovulation in Japanese eel by 17α-OHP and 17α, 20β-DHP Mode, timing and oxytocin effects were observed, the results show that the longer the ovary development, the lower the rate of oxytocin. Cause low rate of oxytocin is mainly due to the Japanese eel in the late stages of oocyte maturation, female individual eel susceptibility to drug-induced large differences in the timing of oxytocin is difficult to grasp. Through the use of transparent liquid oocytes suitable for Japanese eel to observe the occurrence of GVBD, and according to the rate of occurrence of each individual GVBD, to develop appropriate oxytocin solution basically solved the above problems. In this experiment, CP, HCG, 17α-OHP or 17α, 20β-DHP mixed oxytocin were used to increase the average oxytocin yield from 35 4% to 91 9% and 93 0% respectively. The oxytocin amounts for 17α-OHP and 17α, 20β-DHP were 5 mg / (500 g) and 10 mg / (500 g). At (21 ± 0) ℃ water temperature, the time of oxytocin effect was 15 ~ 18h and 13 ~ 16h, respectively.