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甲_2-巨球蛋白(α_2-M)为多种蛋白水解酶的抑制剂,而组织激肽释放酶则是蛋白水解酶的一种。本文研究了两者在正常及辐射损伤大鼠多种组织中的分布及含量(半定量)变化,旨在探索α_2-M治疗辐射烧伤的机理。 材料与方法 Wistar雄性大鼠8只,2只正常,6只经8.5Gy~(60)Co_Υ线照射,于照后第1,3,5天各取2只大鼠的心、肝、脾、肺、肾、睾丸、腮腺、胰腺、胸腺、十二指肠及皮肤,经中性福尔马林固定,低温石蜡包埋,切片(5μm),免疫酶标PAP法染色,普通光镜下观察。 结果 ①在正常大鼠的心、肝、脾、肺、肾、睾丸、腮腺、胰腺、十二指肠及皮肤中的不同部位,均不同
Alpha-2-macroglobulin (α_2-M) is an inhibitor of many proteolytic enzymes, while tissue kallikrein is a proteolytic enzyme. In this paper, we studied the distribution and content (semiquantitative) changes in both tissues of normal and radiation injured rats, and explored the mechanism of α_2-M treatment of radiation burn. MATERIALS AND METHODS: Eight male Wistar rats, two normal and six were irradiated with 8.5Gy ~ (60) Co_Y line. Heart, liver, spleen, The lung, kidney, testis, parotid gland, pancreas, thymus, duodenum and skin were fixed in neutral formalin, embedded in low temperature paraffin, sectioned (5μm) and stained with enzyme-linked immunosorbent assay (PAP) . Results ① In normal rats, heart, liver, spleen, lung, kidney, testis, parotid gland, pancreas, duodenum and skin were all different