Objective This study initially validates the Chinese version of the Physical Activity Questionnaire for Older Children(PAQ-C), which has been identified as a potentially valid instrument to assess moderate-to-vigorous physical activity(MVPA) in children among diverse racial groups. Methods The psychometric properties of the PAQ-C with 742 Hong Kong Chinese children were assessed with the scale’s internal consistency, reliability, test-retest reliability, confirmatory factory analysis(CFA) in the overall sample, and multistep invariance tests across gender groups as well as convergent validity with body mass index(BMI), and an accelerometry-based MVPA. Results The Cronbach alpha coefficient(α=0.79), composite reliability value(ρ=0.81), and the intraclass correlation coefficient(α=0.82) indicate the satisfactory reliability of the PAQ-C score. The CFA indicated data fit a single factor model, suggesting that the PAQ-C measures only one construct, on MVPA over the previous 7 days. The multiple-group CFAs suggested that the factor loadings and variances and covariances of the PAQ-C measurement model were invariant across gender groups. The PAQ-C score was related to accelerometry-based MVPA(r=0.33) and inversely related to BMI(r=-0.18). Conclusion This study demonstrates the reliability and validity of the PAQ-C in Chinese children. Objective This preliminary initially validates the Chinese version of the Physical Activity Questionnaire for Older Children (PAQ-C), which has been identified as a potentially valid instrument to assess moderate-to-vigorous physical activity (MVPA) in children among diverse racial groups. Methods The psychometric properties of the PAQ-C with 742 Hong Kong Chinese children were assessed with the scale’s internal consistency, reliability, test-retest reliability, confirmatory factory analysis (CFA) in the overall sample, and multistep invariance tests across gender groups as well as the convergent validity with body mass index (BMI), and an accelerometry-based MVPA. Results The Cronbach alpha coefficient (α = 0.79), composite reliability value (p = 0.81), and the intraclass correlation coefficient satisfactory reliability of the PAQ-C score. The CFA indicated data fit a single factor model, suggesting that the PAQ-C measures only one construct, on MVPA over the previous 7 days. The multiple-group CFAs suggested that the factor loadings and variances and covariances of the PAQ-C measurement model were invariant across gender groups. The PAQ-C score was related to accelerometry-based MVPA (r = 0.33) and inversely related to BMI = -0.18). Conclusion This study demonstrates the reliability and validity of the PAQ-C in Chinese children.