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结合碳纳米材料和分子印迹技术,建立了以K3[Fe(CN)6]为探针测定盐酸克伦特罗的方法。以邻苯二胺为功能单体,盐酸克伦特罗为模板,采用电化学聚合法在多壁碳纳米管修饰电极表面制备了分子印迹薄膜。用乙腈水溶液可快速去除模板,得到多壁碳纳米管-分子印迹传感器。用循环伏安法、交流阻抗法和石英晶体微天平技术对印迹膜进行了表征,膜厚为12.3 nm。K3[Fe(CN)6]的相对峰电流与盐酸克伦特罗的浓度在4.0×10-8～6.6×10-6 mol/L范围内呈线性关系,检测限为8.1×10-9 mol/L。选择性实验表明传感器对结构类似物具有较强的抗干扰能力。此传感器可用于猪肉中盐酸克伦特罗的测定,加标回收率为101.3%～107.9%。
A method for the determination of clenbuterol using K3 [Fe (CN) 6] as probe was established based on carbon nanomaterials and molecular imprinting techniques. Using o-phenylenediamine as functional monomer and clenbuterol as template, a molecularly imprinted film was prepared on the surface of multi-walled carbon nanotubes modified electrode by electrochemical polymerization. The template was quickly removed with aqueous acetonitrile to obtain a multi-walled carbon nanotube-molecularly imprinted sensor. The imprinted membrane was characterized by cyclic voltammetry, AC impedance and quartz crystal microbalance. The membrane thickness was 12.3 nm. The relative peak current of K3 [Fe (CN) 6] was linear with the concentration of clenbuterol in the range of 4.0 × 10-8 ~ 6.6 × 10-6 mol / L with the detection limit of 8.1 × 10-9 mol / L. Selective experiments show that the sensor has strong anti-interference ability to structural analogues. The sensor can be used for the determination of clenbuterol hydrochloride in pork with the recoveries of 101.3% -107.9%.