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本文用~3H-胸腺嘧啶核苷标记法,研究了大鼠在部分切除大脑皮质后,所出现的分裂神经细胞的来源。实验用成年大白鼠,切除其双侧顶叶正中部大脑皮质,术后立即腹腔注射~3H-胸腺嘧啶核苷,第4天取大脑组织,Carnoy液固定,连续切片,焦油紫染色。结果表明,大脑皮层部分切除后,在损伤区边缘100～200μm范围内的脑组织中,有2～5％的细胞被标记,即细胞核内有银粒分布。其中巨噬细胞、血管内皮细胞和周细胞居多,胶质细胞和神经细胞次之。被标记的细胞绝大部分是分裂间期,少数处在有丝分裂状态。无论那一类细胞,其间期细胞标记得越多,有丝分裂亦多,反之则少,两者成正比,神经细胞亦不例外。因为分裂中的神经细胞有相当一部分具有典型的锥体细胞形态,由此推测,有丝分裂的神经元可能发生于脑皮质原位,由于损伤产生某些物质,引起已经成熟的神经细胞分裂。
In this paper, ~ 3H-thymidine labeling method was used to study the origin of dividing neurons in rats after partial excision of the cerebral cortex. In the experiment, the adult rats were removed from the middle cerebral cortex of both parietal lobes. Immediate intraperitoneal injection of ~ 3H-thymidine was performed. The brain tissue was taken on the fourth day. Carnoy’s liquid was fixed and serially sectioned. The results showed that 2 ~ 5% of the brain cells in the 100 ~ 200μm range at the edge of the lesion were labeled after partial excision of the cerebral cortex, ie, there were silver particles in the nucleus. The majority of macrophages, vascular endothelial cells and pericytes, followed by glial cells and nerve cells. Most of the labeled cells are interphase and a few are in mitosis. No matter what kind of cells, the more the interval of cell markers, mitosis is also more, and vice versa less, the two is proportional to the nerve cells are no exception. Because a considerable part of the dividing nerve cells have typical pyramidal cell morphology, it is speculated that mitotic neurons may occur in situ in the cerebral cortex, due to damage to produce certain substances, causing mature nerve cells to divide.