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应用ＲＴ一ＰＣＲ和基因重组技术，从湖南地区ＨＣＶ感染者血清中获得ＨＣＶＮＳ＿５区的基因克隆。经核苷酸序列的比较分析，此克隆片段属１ｂ型ＨＣＶ基因。应用ＰＭＡＬ－ＣＲＩ质粒在大肠杆菌中高效表达了此基因片段，并获融合蛋白ＭＢＰ－ＨＣＶ·ＮＳ＿５，经ＷｅｓｔｅｒｎＢｌｏｔ分析证实该融合蛋白有较特异的ＨＣＶ抗原性，可用于ＨＣＶ感染的临床诊断和发病机制的研究。
The RT-PCR and gene recombination techniques were used to obtain the cloned HCVNS_5 region from the serum of HCV-infected patients in Hunan Province. The nucleotide sequence of comparative analysis, the cloned fragment is type 1b HCV gene. The fusion protein MBP-HCV · NS_5 was highly expressed in Escherichia coli using PMAL-CRI plasmid. Western blot analysis confirmed that the fusion protein has specific HCV antigenicity and could be used in clinical diagnosis and pathogenesis of HCV infection Mechanism of research.