常规透射电镜制样(超薄切片术)是电镜术中最繁重而又重要的工作,是电镜细胞化学等特殊制样技术的基础,制样效果将直接影响超微结构研究结果的可靠性和重复性。因此,我室近年来更注意在现有条件下改进和提高常规技术,现总结如下。 1.前固定 Conventional transmission electron microscopy (ultra-thin section) is the most burdensome and important work in electron microscopy, is the basis of special sample preparation technology such as electron microscopy cytochemistry, the sample preparation effect will directly affect the reliability of the results of the ultrastructure research and Repeatability. Therefore, our room in recent years pay more attention to improve and enhance the conventional technology under the existing conditions, are summarized as follows. 1. fixed before