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目的观察百草枯(PQ)诱导小鼠脑小胶质(BV2)细胞株的炎症反应。方法取处于对数生长期的BV_2细胞,分别暴露于终浓度为0(阴性对照)、20、40、80μmol/L的PQ溶液继续培养6、12、24、48 h。检测细胞活性和炎症因子[肿瘤坏死因子α(TNF-α)、白细胞介素-6(interleukin 6,IL-6)、IL-1β及活性氧(ROS)]水平。结果与阴性对照组比较,各剂量PQ染毒组BV_2细胞TNF-α、IL-1β、IL-6和ROS的水平及细胞抑制率均较高,差异均有统计学意义(P<0.05);与染毒6 h比较,染毒12、24、48 h时BV_2细胞TNF-α、IL-1β、IL-6和ROS的水平及细胞抑制率均较高,差异均有统计学意义(P<0.05)。且随着PQ染毒剂量的升高和染毒时间的延长,BV_2细胞的抑制率及TNF-α、IL-1β、IL-6和ROS水平均呈上升趋势。结论PQ能活化小鼠小胶质细胞,使其分泌炎症因子,同时诱导氧化损伤,加重细胞毒性作用。
Objective To observe the inflammatory reaction induced by paraquat (PQ) in mouse brain microglia (BV2) cell line. Methods BV_2 cells in logarithmic growth phase were exposed to a final concentration of 0 (negative control). PQ solutions of 20, 40 and 80 μmol / L were cultured for 6, 12, 24 and 48 h, respectively. The cell viability and inflammatory cytokines (TNF-α, IL-6, IL-1β and reactive oxygen species (ROS)) levels were measured. Results Compared with the negative control group, the levels of TNF-α, IL-1β, IL-6 and ROS and the cell inhibitory rate of BV 2 cells in each dose PQ group were significantly higher than those in the negative control group (P <0.05). Compared with the 6 h exposure, the levels of TNF-α, IL-1β, IL-6 and ROS and the cell inhibitory rate of BV 2 cells were higher at 12, 24 and 48 h (P < 0.05). With the increase of PQ dose and exposure time, the inhibitory rate of BV 2 cells and the levels of TNF-α, IL-1β, IL-6 and ROS increased. Conclusion PQ can activate mouse microglial cells to secrete inflammatory factors, induce oxidative damage and aggravate cytotoxicity.