论文部分内容阅读
本文报道了应用表面增强技术将薄层色谱 (TLC)与傅立叶变换拉曼光谱 (FT Raman)联用 ,获得了中草药钩藤中的钩藤碱光谱研究的新方法 .研究表明 ,在薄层原位 2 5μg钩藤总碱 ,即可很好的分离出钩藤碱等多种钩藤生物碱 .应用薄层原位表面增强 (TLC FT SERS)技术 ,获得钩藤碱分子的特征振动谱带 ,进而阐述了样品分子在表面增强基底银胶表面的吸附模式 ,钩藤碱以分子中甲氧基O原子的孤电子对吸附于银晶体微粒表面 ,1397cm- 1的OCH3的变形振动获得最大增强 .说明TLC SERS结合对中草药化学成分进行高灵敏度示踪指纹检测的可靠性和优越性 .
In this paper, we reported a new method for the spectroscopic study of rhynchophylline in Chinese herbal medicine Uncaria by the combination of TLC and Fourier transform Raman spectroscopy (FT-Raman) A total of 2 5 g Uncaria total alkaloids, we can be a good separation of rhynchophylline and other Uncaria alkaloids TLC (thin layer) in situ surface enhancement (TLC FT SERS) technology to obtain the characteristics of the vibration spectrum of rhynchophylline , And then the adsorption pattern of the sample molecules on the surface-enhanced silver substrate was described. Rhynchophylline maximized the deformation vibration of OCH3 at 1397 cm-1 by the lone pair of methoxy O atoms in the molecule adsorbed on the surface of the silver crystal particles The TLC SERS combined with the chemical composition of Chinese herbal medicine for high sensitivity tracer fingerprinting reliability and superiority.