论文部分内容阅读
目的旨在了解西藏酥油中金黄色葡萄球菌携带肠毒素A(sea)毒力基因及脉冲场凝胶电泳(PFGE)分型情况。方法通过聚合酶链式反应(PCR)检测35株酥油来源的金黄色葡萄球菌的sea毒力基因并利用PFGE分型技术对上述金黄色葡萄球菌进行全基因组分析,DNA酶切图谱用Bio Numerics软件进行聚类分析。结果 35株金黄色葡萄球菌中sea毒力基因检出率为8.6%(3株);PFGE图谱聚类分析共得到5大簇和25种PFGE型别。结论建立的金黄色葡萄球菌PFGE分型数据库分子型别较多,对西藏地区酥油中由金黄色葡萄球菌引起的食物中毒的防控与溯源工作具有重要的意义。
The purpose of this study is to understand the genotypes of enterovirus A (sea) virulence genes and pulsed-field gel electrophoresis (PFGE) in Staphylococcus aureus in Tibet butter. Methods The genotypes of 35 strains of butter-derived Staphylococcus aureus were detected by polymerase chain reaction (PCR) and genome-wide analysis was performed on the above Staphylococcus aureus by PFGE typing. The DNA digestion patterns were analyzed by Bio Numerics software Cluster analysis. Results The detection rate of sea virulence gene in 35 strains of Staphylococcus aureus was 8.6% (3 strains). There were 5 large clusters and 25 PFGE types in PFGE map cluster analysis. Conclusion The established PFGE typing database of Staphylococcus aureus has many molecular types, which is of great significance for the prevention and control of food poisoning caused by Staphylococcus aureus in the ghee in Tibet.