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已知子宫肌的功能受血浆卵巢激素的调节,但对血浆与子宫肌的雌二醇(E_2)和孕酮(P)浓度之间关系了解颇少,因此作者用放射免疫法测定43名(27～56岁)正常月经周期、绝经期前后妇女血浆及其子宫肌组织中E_2和P的浓度。其中正常月经周期妇女35人;绝经期前伴月经不规则者3人;绝经期后妇女5人。所有病例均因子宫肌瘤、子宫内膜异位症、月经过多、间断性出血、宫颈浸润前期癌或早期浸润癌以及子宫内膜恶变前期等病变,在全身麻醉下行子宫全切除术,术前采血、分离血浆,作E_2和P的RIA测定。术后从宫体腹侧远离肌瘤的正常组织处切取20×3×8 mm组织块,去除浆膜,称取200～400mg经12酯硫酸钠和NaOH消化,醋酸乙酯提取,经LH-20葡聚扩凝胶柱过滤,以
The function of uterine muscle is known to be regulated by plasma ovarian hormones, but little is known about the relationship between plasma and uterine muscle estradiol (E2) and progesterone (P) concentrations, so the authors measured 43 27 ~ 56 years old) normal menstrual cycle, pre and postmenopausal women plasma and uterine muscle E_2 and P concentrations. Among them, there are 35 women with normal menstrual cycle, 3 with irregular menstruation before menopause and 5 women with postmenopausal women. All cases were due to uterine fibroids, endometriosis, menorrhagia, intermittent bleeding, pre-cervical cancer or invasive early invasive carcinoma and endometrial malignant lesions such as pre-malignancy, hysterectomy under general anesthesia, surgery Before blood collection, plasma separation, for E_2 and P RIA determination. After the operation, the 20 × 3 × 8 mm tissue was excised from the normal tissue of the uterine body far away from the fibroids to remove the serosa, then weighed 200-400 mg digested with sodium 12-sulfate and NaOH and extracted with ethyl acetate. After LH- 20 G poly expansion gel column filtration