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本文提出了分别用暗视野显微技术和透射电子显微技术快速确诊日本对虾杆状核病毒性传染病的方法。在暗视野显微镜下观察到的血淋巴中的病毒粒子大小约为0.5μm;胃表层上皮被病毒感染的细胞核明显地呈白色均质小体,直径10～15μm,球形或椭圆形,坏死细胞被囊化为棕色小块(约20～50μm)。病虾血淋巴和胃经负染制样在透射电子显微镜下观察病毒颗粒大小为400×150nm;核衣壳大小为390×85nm。
In this paper, we propose a rapid method for the detection of tuberculous infection of shrimp by dark field microscopy and transmission electron microscopy. The size of virus particles in the haemolymph observed under a dark-field microscope was about 0.5 μm. The nuclei infected with the virus in the epithelium of the stomach surface were clearly white homogenous bodies with a diameter of 10-15 μm, spherical or oval, and necrotic cells were Encapsulated into small brown pieces (about 20 ~ 50μm). Shrimp shrimp blood and stomach by negative staining samples were observed under a transmission electron microscope virus particle size of 400 × 150nm; nucleocapsid size of 390 × 85nm.