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[目的]研究复方中药糖脂清治疗KK-Ay小鼠糖尿病的作用。[方法]采用高胰岛素血症遗传性2型糖尿病KK-Ay小鼠动物模型。复方中药糖脂清为桑叶、荷叶、丹参等中药材组成,其水煎煮提取物作为实验药物。动物分为4组,分别为模型组,高剂量组(0.7mg/kg),中剂量组(0.35mg/kg),低剂量组(0.175mg/kg);给药方法:1次/d,口服给药,连续给药4周;每周做1次空腹血糖检测,对给药4周后的糖苷酶、总胆固醇、甘油三酯进行检测;计量数据采用均数标准差表示,组间比较采用方差分析,分析软件为Excel和SPSS10.0统计分析软件。[结果]复方中药糖脂清各剂量组,给药前与给药4周后相比各剂量组均可降低血糖,同时降低KK-Ay小鼠小肠的蔗糖酶与麦芽糖酶的活性、血清中总胆固醇和甘油三酯的含量。[结论]复方中药糖脂清具有降低2型糖尿病模型KK-Ay小鼠血糖的作用。其机制为复方中药糖脂清可抑制蔗糖酶和麦芽糖酶活性。同时此方药还具有降低血脂的作用。
[Objective] To study the effect of compound traditional Chinese medicine Tangzhiqing on diabetes in KK-Ay mice. [Methods] The KK-Ay mouse model of hyperinsulinemia inherited type 2 diabetes was used. The compound traditional Chinese medicine glycolipid is composed of Chinese medicinal materials such as mulberry leaf, lotus leaf, and salvia miltiorrhiza, and its water extract is used as experimental medicine. Animals were divided into 4 groups: model group, high-dose group (0.7mg/kg), middle-dose group (0.35mg/kg), low-dose group (0.175mg/kg); administration method: 1 time/d, Oral administration, continuous administration for 4 weeks; weekly fasting blood glucose test, glucosidase, total cholesterol, triglyceride after 4 weeks of administration; measurement data represented by mean standard deviation, comparison between groups Using analysis of variance, the analysis software was Excel and SPSS10.0 statistical analysis software. [Results]Compared with traditional Chinese medicine Tangzhiqing group, before and after administration, compared with those after 4 weeks, blood glucose was reduced in each dose group, and the activities of invertase and maltase in the small intestine of KK-Ay mice were reduced, and serum was reduced. Total cholesterol and triglyceride content. [Conclusion] The compound traditional Chinese medicine Tangzhiqing has the effect of lowering blood glucose in type 2 diabetic model KK-Ay mice. The mechanism is that compound traditional Chinese medicine glycolipid can inhibit sucrase and maltase activity. At the same time this prescription also has the effect of lowering blood lipids.