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目的以天然聚电解质壳聚糖和海藻酸钠为囊材,构建新型的夹层式药物载体脂质体镶囊,并对其结构以及载药和释放性能进行表征。方法以碳酸钙为模板,运用层层自组装技术将二肉豆蔻磷脂酰甘油(DMPG)脂质体包埋在壳聚糖/海藻酸钠形成的囊壁中,制备包含脂质体的聚电解质微囊(脂质体镶囊);以多柔比星作为模型药物,考察多柔比星不同浓度对载药量的影响以及多柔比星从脂质体镶囊中的释放。结果激光共聚焦显微镜和透射电镜显示脂质体成功地包埋在聚电解质微囊的囊壁中,得到分散性良好的脂质体镶囊;随着多柔比星质量浓度(100～1 000μg.mL-1)的增加,脂质体镶囊的载药量呈非线性增加,最高载药量可达30.47×10-3ng;48 h内多柔比星的累积释放百分率约为46%。结论脂质体镶囊具有良好的载药和释放能力,可以作为新型的药物载体深入研究。
OBJECTIVE: To construct a new sandwich-type drug carrier liposomes capsule by using natural polyelectrolyte chitosan and sodium alginate as capsular material, and to characterize its structure, drug loading and release properties. Methods Calcium dihydrogen phosphate (DMPG) liposomes were entrapped in the wall of chitosan / sodium alginate by the layer-by-layer self assembly technique using calcium carbonate as a template to prepare liposome-containing polyelectrolytes Microcapsules (liposomal invagination); with doxorubicin as a model drug to investigate the effect of different concentrations of doxorubicine on drug loading and release of doxorubicin from the liposomal vesicles. Results Laser confocal microscopy and transmission electron microscopy showed that the liposomes were successfully embedded in the cytoplasm of the polyelectrolyte microcapsules to obtain well-dispersed liposomes. With the concentration of doxorubicin (100 ~ 1000μg mL-1), the drug loading of liposomes increased nonlinearly with the highest drug loading of 30.47 × 10-3ng. The cumulative release percentage of doxorubicin in 48h was about 46%. Conclusion Liposomal vesicles have good drug loading and release capacity, which can be used as a new drug carrier in-depth study.