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目的:观察单纯性脑震荡(pure cerebral concussion,PCC)模型大鼠室管膜区小胶质细胞(microglia MG)的反应和时程变化规律,探讨MG、室管膜区和PCC之间的病理关系和意义。方法:采用金属单摆闭合式脑损伤装置制备PCC模型,致伤后随机分为六个损伤组,即3 h、12 h、1 d、2 d、3 d、7 d(n=5),设正常对照组(n=5)。采用免疫组织化学SP法和免疫荧光单标染色法,观察PCC组和正常对照组大鼠脑室管膜区OX-42(MG特异性标记物)的表达变化。结果:正常对照组大鼠脑室管膜区OX-42阳性的小胶质细胞轮廓不清,数量较少,并且OX-42免疫阳性产物的表达很少。PCC组大鼠脑室管膜区OX-42阳性细胞的数量和阳性产物的表达呈现逐渐增高趋势,3 d组达高峰,7 d组有所下降,但仍高于正常对照组。各时间组与正常对照组相比均有显著性差异(P<0.05)。结论:PCC损伤早期大鼠室管膜区的MG出现一系列形态学变化的激活表现,提示脑室管膜区的MG在PCC致伤后的病理变化中扮演着重要角色。
OBJECTIVE: To observe the changes of microglia MG response and time course in the rat model of pure cerebral concussion (PCC) and to explore the pathological changes between MG, ependymal zone and PCC Relationship and meaning. Methods: PCC models were prepared by metal pendulum closed brain injury device and were randomly divided into six injury groups (3 h, 12 h, 1 d, 2 d, 3 d, 7 d, n = 5) The normal control group (n = 5). Immunohistochemical SP method and immunofluorescence single-stained staining were used to observe the expression changes of OX-42 (MG-specific marker) in the peritoneal membrane of rats in PCC group and normal control group. Results: OX-42 positive microglial cells in the ependymal zone of rats in the normal control group were unclear in outline and less in number, and expression of OX-42 immunopositive products was low. The number of OX-42 positive cells and the expression of positive products in the ependymal zone of PCC rats tended to increase gradually, reaching the peak in the 3 d group and decreasing in the 7 d group, but still higher than that in the normal control group. Each time group compared with the normal control group were significantly different (P <0.05). CONCLUSIONS: The morphological changes of MG in the ependymal zone of rats with early PCC injury indicate that MG in the ependymal zone plays an important role in the pathological changes following PCC injury.