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研究GM-CSF在DV、HCV等几种黄病毒DNA疫苗诱导的免疫应答中的作用,并分析其作为黄病毒DNA疫苗佐剂的可能性。构建各种真核表达质粒,抽提质粒DNA,分组免疫小鼠,通过ELISA及间接免疫荧光染色检测小鼠血清抗体的动态水平。DV1及DV2prM/E核酸疫苗与GM-CSF质粒共接种的佐剂组小鼠血清抗体水平低于无佐剂的疫苗组,即GM-CSF显示了一定的免疫抑制作用,其中以DV1prM/E核酸疫苗更为显著;而在HCVC及E1蛋白核酸疫苗中,GM-CSF则具有一定免疫增强作用。GM-CSF作为疫苗佐剂,其作用具有复杂的多样性,因抗原的不同可能会呈现免疫提升或免疫抑制,因此选择其作为核酸疫苗佐剂时需慎重。
To investigate the role of GM-CSF in the immune response induced by several flavivirus DNA vaccines, such as DV and HCV, and to analyze its potential as adjuvant to DNA vaccine of flavivirus. Construction of a variety of eukaryotic expression plasmids, extracted plasmid DNA, group mice immunized by ELISA and indirect immunofluorescence staining to detect serum antibody levels in mice. Serum antibody levels of the adjuvant-treated mice co-vaccinated with the DV1 and DV2prM / E nucleic acid vaccines and the GM-CSF plasmid were lower than those of the unadjuvanted vaccine group, ie, GM-CSF showed a certain immunosuppressive effect with DV1prM / E nucleic acid The vaccine is more significant; while in the HCVC and E1 nucleic acid vaccine, GM-CSF has a certain immune enhancement. GM-CSF as a vaccine adjuvant, its role with a complex diversity, due to the different antigen may show immune enhancement or immunosuppression, so choose as a nucleic acid vaccine adjuvant need to be cautious.