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中国的内脏利什曼病(也称为黑热病)传统上分为平原、山丘和荒漠三个不同类型的疫区,不同疫区的临床表现和流行病学特征有明显差异。这种差异是否可在不同疫区病原体分离株的分子水平上表现出来?如果是的话,这些分子水平的差异可否作为分子标记对不同疫区的分离株加以鉴别?本文总结了20年来本课题组通过动基体DNA、nDNA杂交、PCR-SSCP、RAPD及SSU rDNA可变区和LACK基因序列测定等方法进行的技术,结果显示:来自中国不同疫区的利什曼原虫分离株在分子水平上存在异源性,为不同类型利什曼病的防治对策提供理论依据。
The visceral leishmaniasis (also known as kala-azar) in China has traditionally been divided into three different types of epidemic areas: plains, hills and deserts. The clinical manifestations and epidemiological characteristics of different affected areas are significantly different. Can this difference be demonstrated at the molecular level of pathogen isolates from different affected areas? If so, can these molecular differences be identified as isolates from different affected areas by molecular markers? The results of kinetic DNA, nDNA hybridization, PCR-SSCP, RAPD, SSU rDNA variable region and LACK gene sequencing showed that Leishmania isolates from different epidemic areas in China existed at the molecular level Heterogeneity, to provide a theoretical basis for prevention and treatment of different types of leishmaniasis.