论文部分内容阅读
目的:观察竹节参总皂苷(TSPJ)对脑缺血再灌大鼠神经细胞凋亡和早期快速反应基因c-fos,c-jun表达的影响。方法:60只SD大鼠随机分为假手术组、模型组、尼莫地平组、TSPJ组(200,100,50 mg.kg-1)。线栓法制备大鼠局灶性脑缺血-再灌注损伤模型;HE染色检测脑组织病理变化;TUNEL法检测神经细胞凋亡;免疫组化方法检测c-fos,c-jun的表达。结果:脑缺血再灌注损伤后,模型组大鼠脑组织病理损伤明显,TUNEL细胞较假手术组显著增多(P<0.01),c-fos,c-jun表达较假手术组明显增强(P<0.01);TSPJ可明显改善模型大鼠脑组织病理形态,TSPJ(200,100 mg.kg-1)组与模型组相比,TUNEL阳性细胞数明显减少(P<0.01或P<0.05),c-fos,c-jun阳性表达减少(P<0.01或P<0.05)。结论:对脑缺血损伤具有保护作用,其作用可能是通过下调c-fos,c-jun蛋白表达,从而干预脑缺血后的神经细胞凋亡。
Objective: To observe the effects of total SSP (TSPJ) on neuronal apoptosis and expression of c-fos and c-jun in rats with cerebral ischemia and reperfusion. Methods: Sixty SD rats were randomly divided into sham operation group, model group, nimodipine group and TSPJ group (200,100,50 mg.kg-1). The model of focal cerebral ischemia-reperfusion injury was established by suture method. The pathological changes of brain tissue were detected by HE staining. The apoptosis of nerve cells was detected by TUNEL method. The expressions of c-fos and c-jun were detected by immunohistochemistry. Results: After cerebral ischemia-reperfusion injury, the pathological changes of brain tissue in model group were obvious. TUNEL cells increased significantly (P <0.01), while the expression of c-fos and c-jun increased significantly (P <0.01 or P <0.05). The number of TUNEL positive cells in TSPJ (200,100 mg.kg-1) group was significantly lower than that in model group (P <0.01 or P < fos, c-jun positive expression decreased (P <0.01 or P <0.05). Conclusion: It has the protective effect on cerebral ischemia injury, and its effect may be through the down-regulation of c-fos, c-jun protein expression, thereby intervening the neuronal apoptosis after cerebral ischemia.