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目的:为研究雌激素对血管瘤血管内皮细胞(VEC_3)增殖的影响。方法:选择雌激素受体阳性的婴儿草莓状血管瘤(例1)和混合型血管瘤(例2)各1例,VEC 按组织块法进行培养。例1VEC 传至第3代时,分为5组:组1、2培养液中不加内皮细胞生长支持物(ECGS),组1为对照组,组2加雌二醇(E_2),组3、4、5培养液中加 ECGS,组3为对照组,组4加 E_2,组5加 E_2和羟基三苯氧胺。于培养第3、6、9天作细胞计数和氚标记胸苷掺入检测。结果:第9天的结果示:组1、2相似,VEC 增殖缓慢。组3 VEC 增殖加快,为组1的2倍(P<0.01)。组4 VEC 增殖较组3更旺盛,为后者的2倍(P<0.01)。而组5 VEC 增殖受到抑制,仅处组1水平。例2分组3、4,结果与例1相似。结论:本实验证实在体外环境:①E_2可促进血管瘤 VEC 增殖。②E_2的促增殖作用有赖于某些生长因子参与。③三苯氧胺可抑制 E_2对血管瘤 VEC 的促增殖作用。
Objective: To study the effect of estrogen on the proliferation of vascular endothelial cells (VEC_3) in hemangiomas. METHODS: Estrogen receptor-positive infantile strawberry hemangioma (case 1) and mixed hemangiomas (case 2) were selected in each case. VECs were cultured by tissue block method. Example 1 When VECs were passed to the 3rd generation, they were divided into 5 groups: Group 1 and 2 culture fluids were not supplemented with endothelial cell growth support (ECGS), group 1 was a control group, group 2 was estradiol (E 2 ), group 3 ECGs were added to the culture medium, 4, and 5, and group 3 was the control group, group 4 was added E2, group 5 was added E2 and hydroxy tamoxifen. On the 3rd, 6th and 9th days of culture, cell counts and tritiated thymidine incorporation tests were performed. Results: The results on day 9 show that groups 1 and 2 are similar and VEC proliferation is slow. The proliferation of group 3 VECs was faster than that of group 1 (P<0.01). Group 4 VECs proliferated more than group 3, which was 2 times higher than the latter (P<0.01). However, the proliferation of group 5 VECs was inhibited and was only at group 1 level. Example 2 groups 3 and 4 and the result is similar to Example 1. Conclusion: This experiment confirmed that in vitro: 1E 2 can promote the proliferation of hemangiomas VEC. The proliferation of 2E 2 depends on the participation of certain growth factors. 3 Tamoxifen inhibited the pro-proliferative effect of E_2 on hemangiomas VEC.