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目的通过对神经元及胶质细胞进行HE染色、免疫组化染色,观察GFAP/NSE的阳性表达,探讨推断早期死亡时间(postmortem interval,PMI)的可行性。方法健康白兔36只,分为6组,处死后对兔脑组织取材并进行HE染色及相应免疫组化染色,观察10℃条件下镜下0~24h的变化,分别测定0h、1h、4h、8h、16h、24h阳性细胞的数目和OD值。结果在10℃条件下,HE染色在0~24h内无明显变化;脑组织AST中GFAP的阳性表达在24h内无统计学意义;神经元NSE的表达逐渐降低,并具有统计学意义。结论在10℃条件下NSE的阳性表达与PMI具有相关性,可以用于早期PMI推断;GFAP的阳性表达与早期PMI无相关性。
Objective To investigate the feasibility of inferring the postmortem interval (PMI) by examining the positive expression of GFAP / NSE in neurons and glial cells by HE staining and immunohistochemistry. Methods Thirty-six healthy rabbits were divided into 6 groups. After sacrifice, the brain tissues of rabbits were harvested and stained with hematoxylin and eosin (HE) staining and immunohistochemical staining. The changes of 0 ~ 24h under 10 ℃ were observed. The changes of 0h, 1h, 4h , 8h, 16h, 24h positive cells and OD value. Results There was no significant change of HE staining at 0 ~ 24h in 10 ℃. The positive expression of GFAP in brain tissue was not statistically significant in 24 h. The expression of NSE in neurons decreased gradually and had statistical significance. Conclusion The positive expression of NSE at 10 ℃ is correlated with PMI and can be used for early PMI inference. The positive expression of GFAP has no correlation with early PMI.