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以花粉管通道途径将载有目的基因的外源DNA导入受精胚囊,实现基因转移的技术,已被应用于棉花和水稻育种,开辟了在近期内即可实现分子育种途径。我们于1986年开始在大豆上实施此技术,获得初步结果,变异后代已达D_4代。根椐实验室条件,用改良的提取方法获得了合乎转化要求的DNA,在大豆白花受粉后7—9小时,用微量注射器以3μg/花的量滴在子房上切口处,收得D_0种子,大田种值观察其后代变异情况。供体为野生豆、半野生茶秣食豆和栽培豆里外青;受体是吉林16号和吉林20号栽培品种。共做4个组合,373朵花,得子粒613颗。从439株D_1后代中得15株变异株,频率为3％。在未见变异的其他株的
The introduction of exogenous DNA containing the gene of interest into the fertilized embryo sac by the pollen tube pathway has been applied to cotton and rice breeding and opened the way for molecular breeding in the near future. We started to implement this technology on soybean in 1986, obtaining the preliminary result, the variant offspring has reached D_4 generation. According to the laboratory conditions, DNAs that meet the transformation requirements were obtained by a modified extraction method. After the white soybean powder was pulverized for 7-9 hours, the microtiter was dropped at a dose of 3 μg / Field observations of their offspring variation. The donors were wild beans, semi-wild teas, beans and cultivated beans. The receptors were cultivated in Jilin 16 and Jilin 20. A total of 4 combinations, 373 flowers, 613 seeds. Fifteen mutants were obtained from 439 D_1 offspring at a frequency of 3%. No other strains in the mutation