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为了探讨幽门螺杆菌(HP)感染患者IFN—γ含量、LAK细胞活力与增殖的改变,选择消化性溃疡患者40例,用生物素—亲和素双抗体夹心酶联免疫吸附分析技术(ABC-ELISA)测定外周血清IFN-γ分泌量,用MTT法测定LAK细胞增殖与杀伤活力。结果HP阳性患者IFN—γ含量、LAK细胞增殖与杀伤活力显著低于HP阴性患者(均P<0.01)。提示HP感染患者可能存在细胞免疫功能低下。
In order to investigate the changes of IFN-γ content and LAK cell viability and proliferation in patients with Helicobacter pylori (HP) infection, 40 patients with peptic ulcer were selected and treated with biotin-avidin double antibody sandwich enzyme-linked immunosorbent assay (ABC- ELISA) was used to measure the secretion of IFN-γ in peripheral blood. The proliferation and cytotoxicity of LAK cells were measured by MTT assay. Results The content of IFN-γ and the proliferation and cytotoxicity of LAK cells in HP positive patients were significantly lower than those in HP negative patients (all P <0.01). Tip HP infected patients may have cellular immune dysfunction.