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铁皮石斛不仅是著名的兰科观赏花卉,而且也是名贵的中药材之一,具有很高的药用价值。野生的铁皮石斛濒临灭绝,保护和挽救工作意义重大。本文采用植物组织培养方法对其进行研究,探索和总结出可实用的方法。以MS(改良)培养基附加6-BA4.0mg/L+NAA0.5mg/L+IBA0.3mg/L+0.3%蔗糖+6.5g/L琼脂,pH值5.6,作为分化增殖培养基,30天后每株产生出健壮的丛生芽5-6个,将其丛生芽分株转接到1/2MS(改良)培养基中,10天开始生根3,0天生根率90%以上,茎高5-6cm,为炼苗移栽和应用推广提供重要的科学依据。
Dendrobium is not only famous orchid ornamental flowers, but also one of the precious Chinese herbal medicines, with high medicinal value. Wild Dendrobium verge of extinction, conservation and rescue work of great significance. In this paper, plant tissue culture methods to study it, explore and summarize practical methods. 6-BA 4.0 mg / L + NAA 0.5 mg / L + IBA 0.3 mg / L + 0.3% sucrose + 6.5 g / L agar was added to MS (modified) medium at a pH of 5.6 as a differentiation proliferation medium and after 30 days Each cluster produced 5-6 robust clusters of shoots, transferred their cluster shoots to 1/2 MS (modified) medium, rooted at 10 days, rooted at 3,0 days and over 90% 6cm, for the Miao Miao transplanting and application of promotion to provide an important scientific basis.