目的　制备抗人cTnⅠ单克隆抗体 ,以便进一步制备AMI诊断制剂。方法　用兔骨骼肌Tnc(sTnC)制备TnC Sepharose 4B亲和柱 ,直接从心肌组织中分离提纯人cTnⅠ。结果　所提纯的cTnⅠ经SDS PAGE分析已达电泳纯 ,其相对分子质量和氨基酸组成与文献报道相符。结论　为建立AMI早期诊断试剂奠定了基础。 Objective To prepare anti-human cTn I monoclonal antibodies for the further preparation of AMI diagnostic preparations. Methods The TnC Sepharose 4B affinity column was prepared with rabbit Tnc (sTnC), and the human cTn Ⅰ was isolated and purified directly from the myocardium. Results The purified cTnⅠwas electrophoresed pure by SDS PAGE. The relative molecular mass and amino acid composition of the purified cTnⅠwere consistent with those reported in the literature. Conclusions for the establishment of early diagnosis of AMI lays the foundation.