将8天鸡胚大脑皮层制成单细胞悬液,进行静止培养。若接种于鼠尾胶原上,并于第5天加用药物以抑制有丝分裂,可见仍有大量胶质细胞生长。神经细胞在总细胞数中不足70％,三周后开始退化。若接种于聚赖氨酸处理过的玻片上,则胶质细胞很少出现,神经细胞占总细胞数的80％以上,但两周后开始退化。因后一方法可以基本上排除胶质细胞的干扰,故适用于单独观察神经细胞的研究工作。 The 8-day-old chick embryo cerebral cortex was made into a single cell suspension for static culture. If inoculated on the murine collagen, and add drugs on the 5th day to inhibit mitosis, we can see that there is still a large number of glial cells. Nerve cells in less than 70% of the total cell number, began to degenerate after three weeks. When inoculated on polylysine-treated slides, glial cells rarely appeared, and neurons accounted for more than 80% of the total number of cells, but degenerated after two weeks. Because the latter method can basically rule out the interference of glial cells, it is suitable for the observation of nerve cells alone.