论文部分内容阅读
本文首次采用超声波法把烟草花叶病毒（ＴＭＶ）粒子导入带壁烟草细胞，建立病毒—细胞高效侵染体系。将烟草细胞置于含ＴＭＶ粒子的缓冲液中，用声强为０．５Ｗ／ｃｍ２的脉冲超声波处理５ｍｉｎ，培养４８ｈ后使用荧光免疫法检测烟草细胞转染率为５９．７％。经酶联免疫吸附测定（ＥＬＩＳＡ）检测，ＴＭＶ粒子在烟草细胞体内增殖４８ｈ后达到高峰。电镜观察细胞体内有大量的ＴＭＶ粒子。用枯斑寄主法测定表明ＴＭＶ子代有较高的致病力。
In this paper, the tobacco mosaic virus (TMV) particles were introduced into tobacco-bearing tobacco cells by ultrasonic method for the first time to establish virus-cell efficient infection system. The tobacco cells were placed in buffer containing TMV particles and treated with pulsed ultrasound with a sound intensity of 0.5 W / cm2 for 5 min. After 48 h incubation, the transfection rate of tobacco cells was 59.7% by fluorescence immunoassay. The results of enzyme-linked immunosorbent assay (ELISA) showed that the TMV particles peaked in tobacco cells 48h after proliferation. Electron microscopy of cells with a large number of TMV particles. The test of dead-leaf host showed that TMV progeny had higher virulence.