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以新鲜猪脊髓制备运动神经元（ＳＭＮ）接种豚鼠或直接用新鲜猪脊髓前角匀浆（ＳＡＨ）接种Ｌｅｗｉｓ大鼠或Ｗｉｓｔａｒ大鼠。豚鼠在第五次接种后，Ｌｅｗｉｓ大鼠在第一次或二次接种后和Ｗｉｓｔａｒ大鼠经第三次接种后，出现程度不同的后肢无力、瘫痪、体重下降，组织切片检查发现脊髓前角运动神经元变性和丧失，经免疫组化染色阳性证明血清中有抗运动神经元特异性抗体，对照组动物则均无任何症状和组织病理改变，血清抗体阴性。实验结果表明，用纯化或未纯化的猪运动神经元为抗原，可建立豚鼠或大鼠免疫型运动神经元病（ＭＮＤ）动物模型，说明猪与豚鼠、猪与大鼠运动神经元抗原有共同结构。用前角接种者较纯运动神经元接种者出现症状快，而Ｌｅｗｉｓ大鼠出现症状最快和发病率最高，Ｗｉｓｔａｒ大鼠出现症状次之。
Mice were inoculated with fresh porcine spinal cord motor neurons (SMN) or Lewis rats or Wistar rats were inoculated directly with fresh pig spinal anterior horn homogenate (SAH). Guinea pigs After the fifth vaccination, Lewis rats developed hindlimb weakness, paralysis and weight loss after the first or second inoculation and the third inoculation of Wistar rats. Histological examination revealed that spinal cord anterior horn Degeneration and loss of motor neurons, positive by immunohistochemical staining serum anti-motor neurons specific antibodies, the control group of animals without any symptoms and histopathological changes, serum antibody negative. The experimental results show that the guinea pig or rat immune motor neuron disease (MND) animal model can be established by using the purified or unpurified porcine motor neuron as antigen, which shows that pigs are common to guinea pig, pig and rat motor neuron antigens structure. Symptoms were faster in vaccinated subjects with anterior horn than in pure motor neurons, whereas Lewis rats had the fastest and the highest incidence of symptoms, followed by symptoms in Wistar rats.