论文部分内容阅读
目的观察二十二碳六烯酸(DHA)对人胰腺癌细胞株生长的抑制作用。方法将DHA与胰腺癌细胞混合培养,采用MTT、流式细胞技术分析细胞增殖、细胞凋亡、细胞周期以及凋亡相关蛋白Bcl-2含量。结果 50μg/mlDHA作用人胰腺癌细胞株后,Patu8988、SW1990增殖率24h为(46.89±5.99)、(46.03±7.69),48h为(35.92±2.91)、(25.70±5.60),肿瘤细胞增殖受到明显抑制(P<0.01)。同时细胞凋亡率明显升高,效应呈现时间-剂量依赖关系,72h后Patu8988、SW1990凋亡率分别为53.2%、13.7%。G0～G1期细胞比例增高,S期细胞比例下降。DHA作用24h后,Patu8988、SW1990Bcl-2蛋白表达率明显低于对照组(P<0.05)。结论 DHA能抑制胰腺癌细胞增殖,并通过下调Bcl-2在细胞中表达来诱导胰腺癌细胞凋亡。
Objective To observe the inhibitory effect of docosahexaenoic acid (DHA) on the growth of human pancreatic cancer cell lines. Methods DHA and pancreatic cancer cells were mixed and cultured. Cell proliferation, apoptosis, cell cycle and apoptosis related protein Bcl-2 were analyzed by MTT and flow cytometry. Results The proliferation rates of Patu8988 and SW1990 cells after treated with 50μg / ml DHA for 48 h were (46.89 ± 5.99), (46.03 ± 7.69) and (35.92 ± 2.91), (25.70 ± 5.60), respectively. The proliferation of tumor cells was significantly increased Inhibition (P <0.01). At the same time, the apoptosis rate was significantly increased. The effect showed a time-dose-dependent relationship. After 72h, the apoptosis rates of Patu8988 and SW1990 were 53.2% and 13.7%, respectively. G0 ~ G1 phase cells increased, S phase cells decreased. After DHA for 24 hours, the expression of Patu8988 and SW1990Bcl-2 protein was significantly lower than that of the control group (P <0.05). Conclusion DHA can inhibit the proliferation of pancreatic cancer cells and induce the apoptosis of pancreatic cancer cells by down-regulating the expression of Bcl-2 in the cells.