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我们前期研究表明运动性和高血压性心肌肥大细胞表型变化在结构、功能和代谢方面均表现不同，但两者基因表达的不同特征尚不清楚。本实验采用Northern分子杂交方法对游泳运动12周大鼠和自发性高血压大鼠（spontaneouslyhypertensiverat,SHR）肥大心脏心肌初级和次级应答基因表达进行比较研究。结果表明，游泳大鼠心系数比对照大鼠提高26％（P＜0．01），心肌c－fos和心房钠尿肽（atrialnatriuretic，ANF）基因表达在最后一次运动后即刻明显增强，在运动后24h的稳定期，c－fos基因表达降低，但仍高于对照大鼠，而ANF基因表达则降低至对照大鼠水平；肌球蛋白α重链（myosinheavychain，MHC）基因表达增强，α／β-MHC基因表达比值提高。SHR心系数比对照大鼠提高61％（P＜0．01），心肌c-fos和ANF基因表达呈持续增强，β-MHC基因表达增强，α／βMHC基因表达比值降低。这些结果说明，运动性和高血压性心肌肥大重塑时心肌的初级和次级应答基因表达特征不同。本文提示：（1）c-fos等原癌基因的不同表达可能对两种心肌肥大的重塑具有不同的调节作用；（2）ANF基因的不同表达可作为在转录水平上区别两种不同心肌肥大的分子标志；（3）α／β-MHC基因比值的不同表达可在转录水平上调节运动性和高血压性肥大心脏?
Our previous studies showed that the phenotypic changes of exercise-induced and hypertensive cardiac myocytes differed in their structure, function and metabolism, but the different characteristics of the gene expression between the two were unclear. In this study, Northern blot hybridization was used to compare the gene expression of primary and secondary responses in hypertrophic myocardium of swimming rats of 12 weeks and spontaneously hypertypertensive rats (SHR). The results showed that the heart coefficient of swimming rats was increased by 26% (P <0.01), the expression of c-fos and atrial natriuretic (ANF) genes in myocardium significantly increased immediately after the last exercise. The expression of c-fos gene was lower than that of control rats at the stable phase of 24h, but the ANF gene expression was decreased to the level of control rats. The expression of myosin-heavy chain (MHC) β-MHC gene expression ratio increased. The cardiac index of SHR was increased by 61% (P <0.01), the expression of c-fos and ANF in myocardium was continuously increased, the expression of β-MHC gene was enhanced and the ratio of α / βMHC gene expression was decreased. These results suggest that primary and secondary response genes differ in their cardiac remodeling during exercise and hypertensive cardiac hypertrophy. This article suggests that: (1) Different expression of proto-oncogenes such as c-fos may have different regulatory effects on the remodeling of both cardiac hypertrophy; (2) The different expression of ANF gene can be used as a marker for differentiating two different cardiac types at the transcriptional level Hypertrophy of molecular markers; (3) α / β-MHC gene ratio of different expression can regulate motor activity and hypertensive hypertrophy at the transcriptional heart?