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用ＤＮＡ复性动力学方法克隆到一个水稻（ＯｒｙｚａｓａｔｉｖａＬ．）中度重复ＤＮＡ顺序。不同限制性内切酶消化和Ｓｏｕｔｈｅｒｎ杂交分析显示，这段重复ＤＮＡ顺序以串联加散布的形式存在于水稻基因组中；序列分析表明在它内部含有一个典型的植物启动子序列ＴＧＴＡＴＡＡＡＴＡ；以ｐＲＲＤ３克隆片段作探针，对水稻３４个品种进行拷贝数测定，在野生稻与栽培稻、籼稻与粳稻之间均存在拷贝数上的明显差异；对ＡＡ基因组不同亚型水稻ＤＮＡ进行Ｓｏｕｔｈｅｒｎ杂交分析，得到基因组亚型特异的杂交带谱，说明该重复顺序是研究水稻进化和分类的一个有用探针。
Cloning of a rice (Oryzasativa L.) moderately repeated DNA sequence by DNA refolding kinetics. Different restriction endonuclease digestion and Southern hybridization analysis showed that this repetitive DNA sequence existed in the rice genome in a series and scattered manner. Sequence analysis showed that it contained a typical plant promoter sequence TGTATAAATA, pRRD3 cloning fragment As a probe, the copy number of 34 varieties of rice were measured, there was a significant difference in copy number between wild rice and cultivated rice, indica and japonica rice; Southern hybridization analysis of rice genotypes with different subtypes of rice genomic Subtype-specific hybridization bands indicate that this repetitive sequence is a useful probe for studying rice evolution and taxonomy.