论文部分内容阅读
目的 研究血管紧张素转化酶 (ACE)基因多态性在慢性肾炎 (CGN)及原发性肾病综合征 (PNS)患者的分布与临床表现的关系。方法 提取外周血DNA ,然后PCR扩增基因片段 ,检测 86例慢性肾炎慢性肾功能不全 (尿毒症期 )患者 (CNG组 )、5 6例PNS患者 (PNS组 )及 5 0例健康献血员 (对照组 )的ACE基因多态性。结果 CGN组插入纯合子(II)基因型较对照组比例高 ,女性患者缺失杂合子 (DI)发生率上升 ,缺失纯合子 (DD)发生率下降。高血压发生率由大到小依次是DD型 >DI型 >II型 ,而血中ACE浓度也是DD型 >DI型 >II型 ,提示缺失 (D)基因产物ACE浓度与高血压呈正相关关系。PNS组DD型发生率较CGN组及对照组高 ,其中PNS组与CGN组对比差异有显著性 (P <0 0 1)。结论 CGN及PNS患者的临床表现与ACE基因多态性有关
Objective To investigate the relationship between the distribution of angiotensin converting enzyme (ACE) gene polymorphisms and the clinical manifestations in patients with chronic nephritis (CGN) and primary nephrotic syndrome (PNS). Methods The peripheral blood DNA was extracted and then the gene fragments were amplified by PCR. 86 cases of chronic renal failure (CNG group), 56 cases of PNS (PNS group) and 50 healthy blood donors Control group) ACE gene polymorphism. Results The genotype of homozygote (II) inserted into CGN group was higher than that of control group. The incidence of DI in female patients was increased and the incidence of DD was decreased. The incidence of hypertension was descending order of DD type> DI type> II type, while the concentration of ACE in blood was also DD type> DI type> II type, suggesting that there was a positive correlation between ACE gene concentration and hypertension. The incidence of DD in PNS group was higher than that in CGN group and control group, and there was significant difference between PNS group and CGN group (P <0.01). Conclusions The clinical manifestations of CGN and PNS are related to ACE gene polymorphism