论文部分内容阅读
目的通过对日常食品中分离的金黄色葡萄球菌产肠毒素情况的研究,为今后金黄色葡萄球菌食品中毒的预防和控制提供依据。方法采用GB 4789.10—2010附录B葡萄球菌肠毒素检验方法对120株金黄色葡萄球菌进行检测。结果 120株金黄色葡萄球菌检出产肠毒素55株,阳性率为45.8%,产生2种或2种以上肠毒素的菌株有38株,占69.1%,产肠毒素的菌株中产肠毒素SEA的有41株,占74.5%。动物源性食品、豆制品,冷冻饮品、糕点和茶制品分离株的肠毒素阳性率分别为62.5%、5.9%、22.2%、12.5%和16.7%,不同来源分离株产肠毒素阳性率差异有统计学意义(χ~2=28.3,P<0.05)。结论动物源性食品产肠毒素是此次5类食品中最高的,需要特别注意并预防该类食品因肠毒素引起的食物中毒,同时本研究对临床给药治疗也具有重要的指导意义。
Objective To study the situation of enterotoxins produced by Staphylococcus aureus isolated from daily food and provide evidence for the prevention and control of Staphylococcus aureus food poisoning in the future. Methods Staphylococcus aureus (120 Staphylococcus aureus) was tested by staphylococcal enterotoxin test in Appendix B of GB 4789.10-2010. Results 120 strains of Staphylococcus aureus were found to produce 55 enterotoxigenic strains, the positive rate was 45.8%. There were 38 strains producing 2 or more enterotoxins, accounting for 69.1%, and the enterotoxigenic strains producing enterotoxin SEA There are 41 strains, accounting for 74.5%. The positive rates of enterotoxins in the isolates of animal-derived foods, soy products, frozen drinks, pastries and tea products were 62.5%, 5.9%, 22.2%, 12.5% and 16.7%, respectively Statistical significance (χ ~ 2 = 28.3, P <0.05). Conclusion The production of enterotoxins from animal-derived foods is the highest among the five types of foodstuffs. It is necessary to pay special attention to and prevent food poisoning caused by enterotoxins in these foods. This study also has important guiding significance for the clinical administration.